Development and characterization of polymorphic EST-SSR markers forPaphiopedilum henryanum(Orchidaceae)
نویسندگان
چکیده
منابع مشابه
Development and Characterization of Polymorphic EST-SSR and Genomic SSR Markers for Tibetan Annual Wild Barley
Tibetan annual wild barley is rich in genetic variation. This study was aimed at the exploitation of new SSRs for the genetic diversity and phylogenetic analysis of wild barley by data mining. We developed 49 novel EST-SSRs and confirmed 20 genomic SSRs for 80 Tibetan annual wild barley and 16 cultivated barley accessions. A total of 213 alleles were generated from 69 loci with an average of 3....
متن کاملDevelopment and characterization of 29 polymorphic EST-SSR markers for Stipa purpurea (Poaceae)1
PREMISE OF THE STUDY Expressed sequence tag-simple sequence repeat (EST-SSR) markers were developed using Illumina sequencing for further genetic diversity studies of Stipa purpurea (Poaceae). METHODS AND RESULTS Twenty-nine polymorphic and eight monomorphic EST-SSR loci were developed and characterized in 90 individuals from nine S. purpurea populations. The number of alleles per locus range...
متن کاملDevelopment of Novel Polymorphic EST-SSR Markers in Bailinggu (Pleurotus tuoliensis) for Crossbreeding
Identification of monokaryons and their mating types and discrimination of hybrid offspring are key steps for the crossbreeding of Pleurotus tuoliensis (Bailinggu). However, conventional crossbreeding methods are troublesome and time consuming. Using RNA-seq technology, we developed new expressed sequence tag-simple sequence repeat (EST-SSR) markers for Bailinggu to easily and rapidly identify ...
متن کاملDevelopment of 23 novel polymorphic EST-SSR markers for the endangered relict conifer Metasequoia glyptostroboides1
PREMISE OF THE STUDY Metasequoia glyptostroboides is an endangered relict conifer species endemic to China. In this study, expressed sequence tag-simple sequence repeat (EST-SSR) markers were developed using transcriptome mining for future genetic and functional studies. METHODS AND RESULTS We collected 97,565 unigene sequences generated by 454 pyrosequencing. A bioinformatics analysis identi...
متن کاملDevelopment of EST-SSR markers of Ipomoea nil
Although Japanese morning glory (Ipomoea nil (L.) Roth.) has been used intensively for genetic studies, DNA markers have not been developed in Ipomoea nil sufficient to cover all chromosomes. Therefore, we conducted microsatellite (simple sequence repeats, SSR) marker development in I. nil for future genetic studies. From 92,662 expressed sequence tag (EST) sequences, 514 unique microsatellite-...
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ژورنال
عنوان ژورنال: Applications in Plant Sciences
سال: 2018
ISSN: 2168-0450
DOI: 10.1002/aps3.1152